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Stomatologija 2009; 11 (1): 21-25 215 KB

In vitro study of microbial leakage in roots filled with EndoREZ sealer/EndoREZ® Points and AH Plus sealer/conventional
gutta-percha points

Saulius Drukteinis, Vytaute Peciuliene, Rasmute Maneliene, Ruta Bendinskaite

Summary

Objective. The aim of this study was to determine and compare the microbial leakage of roots filled with EndoREZ sealer/EndoREZ® Points and AH Plus sealer/ conventional gutta-percha points.

Materials and methods. 60 single-rooted teeth were prepared using step-back technique. The smear layer was removed with 18% EDTA. Teeth were divided into two experimental groups (n=25 each group) and two controls (n=5 each group). In AH Plus group root canals were obturated with AH Plus sealer/gutta-percha and in EndoREZ group with EndoREZ sealer/EndoREZ® Points. Five teeth were served as negative controls and five as positive controls. All teeth were inserted into Eppendorf plastic tubes and suspended in glass bottles containing sterile Schaedler broth. The coronal chambers were filled with the mix of human saliva and broth (ratio 3:1). The medium was changed every 7 days. Microbial growth in the broth was evaluated every day up to the end of experiment.

Results. Leakage in the root canals of the teeth from experimental groups occurred between 4 and 75 days. The mean leakage in AH Plus group was 18.86 days, while in EndoREZ group it was 28.28. No statistically significant difference in microbial leakage between two tested filling materials was found.

Conclusion. Both types of root fillings – EndoREZ sealer/EndoREZ® Points and AH Plus sealer/gutta-percha points – showed microbial leakage.

Key words: AH Plus, EndoREZ, microbial leakage, root canal filling.

Received: 22 12 2008

Accepted for publishing: 26 03 2009

*Institute of Odontology Faculty of Medicine Vilnius university

Saulius Drukteinis* – D.D.S, PhD, lecturer

Vytaute Peciuliene* – D.D.S, PhD, assoc. prof.

Rasmute Maneliene* – D.D.S, PhD, assoc. prof.

Ruta Bendinskaite* – D.D.S, PhD, lecturer

Address correspondence to assist. prof. Saulius Drukteinis, Institute of Odontology, Faculty of Medicine, Vilnius university, Zalgirio 117, 08217, Vilnius.

E-mail address: saulius.drukteinis@gmail.com



	Baltic Dental and Maxillofacial Journal

March, 2009, Vol. 11, No. 1 CONTENTS ABSTRACTS The First International Baltic Ossoeintegration Academy (BOA) Congress "Thoughtful Look to the Nowadays Problems in Oral Implantology. 12-13 June 2009, Kaunas, Lithuania REVIEWS Attitudes of general dental practitioners towards endodontic standarts and adoption of new technology: Literature review Vytaute Peciuliene, Rasmute Maneliene, Saulius Drukteinis, Jurate Rimkuviene 11-14 SCIENTIFIC ARTICLES Three-dimensional assessment of musculoskeletal features in Class II and Class III patients Katrina Zepa, Ilga Urtane, Zane Krisjane, Gaida Krumina 15-20 In vitro study of microbial leakage in roots filled with EndoREZ sealer/EndoREZ® Points and AH Plus sealer/conventional gutta-percha points Saulius Drukteinis, Vytaute Peciuliene, Rasmute Maneliene, Ruta Bendinskaite 21-25 REVIEWS Occlusal features and masticatory muscles activity. A review of electromyographic studies Francesca Trovato, Bruno Orlando, Mario Bosco 26-31 SCIENTIFIC ARTICLES Three-dimensional evaluation of TMJ parameters in Class II and Class III patients Zane Krisjane, Ilga Urtane, Gaida Krumina, Katrina Zepa 32-36 CLINICAL CASE REPORTS Accessory parotid gland carcinoma ex pleomorphic adenoma. Case study diagnosed by fine needle aspiration D. Tamiolakis, T. S. Chimona, G. Georgiou, E. Proimos, S. Nikolaidou, G. Perogamvrakis, C. E. Papadakis 37-40 © 2009 Stomatologija		Stomatologija 2009; 11 (1): 21-25 215 KB In vitro study of microbial leakage in roots filled with EndoREZ sealer/EndoREZ® Points and AH Plus sealer/conventional gutta-percha points Saulius Drukteinis, Vytaute Peciuliene, Rasmute Maneliene, Ruta Bendinskaite Summary Objective. The aim of this study was to determine and compare the microbial leakage of roots filled with EndoREZ sealer/EndoREZ® Points and AH Plus sealer/ conventional gutta-percha points. Materials and methods. 60 single-rooted teeth were prepared using step-back technique. The smear layer was removed with 18% EDTA. Teeth were divided into two experimental groups (n=25 each group) and two controls (n=5 each group). In AH Plus group root canals were obturated with AH Plus sealer/gutta-percha and in EndoREZ group with EndoREZ sealer/EndoREZ® Points. Five teeth were served as negative controls and five as positive controls. All teeth were inserted into Eppendorf plastic tubes and suspended in glass bottles containing sterile Schaedler broth. The coronal chambers were filled with the mix of human saliva and broth (ratio 3:1). The medium was changed every 7 days. Microbial growth in the broth was evaluated every day up to the end of experiment. Results. Leakage in the root canals of the teeth from experimental groups occurred between 4 and 75 days. The mean leakage in AH Plus group was 18.86 days, while in EndoREZ group it was 28.28. No statistically significant difference in microbial leakage between two tested filling materials was found. Conclusion. Both types of root fillings – EndoREZ sealer/EndoREZ® Points and AH Plus sealer/gutta-percha points – showed microbial leakage. Key words: AH Plus, EndoREZ, microbial leakage, root canal filling. Received: 22 12 2008 Accepted for publishing: 26 03 2009 Institute of Odontology Faculty of Medicine Vilnius university Saulius Drukteinis – D.D.S, PhD, lecturer Vytaute Peciuliene* – D.D.S, PhD, assoc. prof. Rasmute Maneliene* – D.D.S, PhD, assoc. prof. Ruta Bendinskaite* – D.D.S, PhD, lecturer Address correspondence to assist. prof. Saulius Drukteinis, Institute of Odontology, Faculty of Medicine, Vilnius university, Zalgirio 117, 08217, Vilnius. E-mail address: saulius.drukteinis@gmail.com