Baltic Dental and Maxillofacial Journal
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2003, Vol. 5, No. 4



Restorative Factors that Affect the Biomechanics of the Dental Implant


The Influence of Sodium Fluoride on Chemiluminescence of Neutrophilic Leukocytes

Evaluation of Dental Health of Dental Students at Kaunas University of Medicine

Bone Mineral Density and Radiographic Mandibular Body Height

An in vitro study of the Sealing Ability of Materials Used for Furcation Perforation Treatment

Factors influencing the health of periodontal tissue and intensity of dental caries

Status of Individual Dosimetry for Dentists in Lithuania in year 1996-2001


An Atraumatic Technique for Fabricating Precise Impressions

© 2004 Stomatologija

Stomatologija 2003; 5 (4): 129-32 219 KB

The Influence of Sodium Fluoride on Chemiluminescence of Neutrophilic Leukocytes

Ingrida Pacauskiene, Nomeda Baseviciene, Pajauta Paipaliene,
Jonas Zekonis


In spite of wide use of fluoridated dental products in dentistry, until now there are no available data on the influence of fluoride (F-) on oxidative activity of neutrophil leucocytes, which play the major role in protecting periodontal tissues and oral mucosa from pathogens. The aim of the present study was to investigate and compare the effect of sodium fluoride on the oxidative activity of venous blood neutrophils of the periodontally healthy control subjects (n=53) and patients with chronic periodontitis (n=52).

Luminol-dependent chemiluminescence was measured after incubation of the neutrophils with NaF (5, 10 and 10 µM, 7.5 and 15 mM F-) and activation with nonopsonized E.coli and opsonized zymosan.

The level of chemiluminescence of the neutrophils depended on concentration of NaF in the incubation medium and the state of periodontal tissues. Low concentration of fluoride (5 µM and 10 µM F-) had no influence on the chemiluminescence. Luminol-dependent chemiluminescence of the non activated and activated neutrophils reliably increased at a concentration of 50 µM F-. At concentrations of 7.5 and 15 mM F-, chemiluminescence of non activated neutrophils in both the studied groups reliably increased, whereas chemiluminescence of the activated neutrophils decreased. At concentrations of 7.5 and 15 mM F-, the level of chemiluminescence of non activated neutrophils was reliably higher in patients with periodontitis than that of healthy subjects.

According to the data of this study, NaF effecting the production of reactive oxygen species by neutrophilic leucocytes may influence the protection periodontal tissues from pathogenic microorganisms.

Key words: neutrophilic leucocytes, luminescence, fluoride, reactive oxygen species, periodontitis.

Address correspondence to Dr.Ingrida Pacauskiene, Clinic of Dental and Oral Pathology, Eiveniu 2, Kaunas, Lithuania.

Received: 22 10 2003

Accepted for publishing: 27 11 2003