|Baltic Dental and Maxillofacial Journal|
2003, Vol. 5, No. 3
© 2003, Stomatologija
Stomatologija 2003; 5 (3): 90-92 188 KB
Pecularities of Generation of Superoxide Anion of Peripheral Blood Leukocytes in Periodontitis
Jonas Zekonis, Gediminas Zekonis, Renata Sadzeviciene, Juozas Zilinskas
The aim of the present study was to explore oxidative function of periodontitis patients and healthy subjects blood neutrophils stimulated with opsonized zymosan, nonopsonized E.coli and E.coli LPS by method of lucigenin-dependent chemiluminescence.
The leukocytes for present investigation were obtained from peripheral venous blood of 16 periodontitis patients and 10 healthy subjects. Superoxide anions production was measured by a lucigenin-enhanced chemiluminescence assay.
Lucigenin-dependent chemiluminescence stimulated with opsonized zymosan, nonopsonized E.coli and E.coli LPS neutrophils of peripheral blood (1361±169, 2422±337 and 4073±893 cpm respectively) was significantly higher than analogous chemiluminescence of control subjects (492±56, 651±103 ir 381±67 cpm respectively).
The lucigenin-dependent chemiluminescence of nonstimulated PMN of periodontitis patients (390±65 cpm) was significantly higher (p ≤ 0,001) than analogous of control subjects (137±13 cpm).
The results indicate, that oxidative function of neutrophils of periodontitis patients was increased.
Keywords: periodontitis, neutrophils, chemiluminescence, lucigenin.
Address correspondence to prof. Jonas Zekonis, Sukileliu 51, Kaunas, Lithuania.
Received: 22 08 2003
Accepted for publishing: 27 09 2003