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Stomatologija 2011; 13 (4): 117-122 454 KB

Specific signaling molecule expression in periodontal ligaments in different age groups: pilot study

Maris Grzibovskis, Ilga Urtane, Mara Pilmane

Summary

Introduction. Orthodontic teeth movement is accompanied by remodeling of alveolar bone, including the interradicular septum and periodontal ligaments (PDL). Periodontal signaling molecules have important functions during tooth movement and they are active in the bone remodeling process. Patients involved in orthodontic treatment belong to different age groups: therefore age must be considered as a contributing factor compromising the remodeling potential of periodontal tissues. The aim of the current study was to investigate the specific expression of signaling molecules in the PDL of interradicular septum in patients from different groups of age.

Materials and methods. The study group included 25 patients to whom extractions of teeth was recommended as a part of further orthodontic treatment. 25 patients (10 males and 15 females) were divided into three groups as follows: 1) 12-14 years old; 2) 15-22 years old; and 3) 23 years old or older. The routine histological method was followed and samples were stained with hematoxyline-eosine. According to literature data in current immunohistochemical study were included and examined expression of NGFR (nerve growth factor receptor), TGF-β (transforming growth factor β), bFGF (basic fibroblast growth factor), FGFR1 (fibroblast growth factor receptor), IL-1 (interleukin 1), IL-6 (interleukin 6), IL-8 (interleukin 8), MMP-1 (matrix metalloproteinase 1), MMP-2 (matrix metalloproteinase 2), MMP-8 (matrix metalloproteinase 8), MMP-9 (matrix metalloproteinase 9), MMP-13 (matrix metalloproteinase 13) in PDL of interradicular septum. The distribution of these factors was evaluated semi quantitatively.

Results. Expression levels of FGFR1, bFGF, MMP 8 and 9, and IL-6 in PDL of interradicular septum structure were determined in all samples. Decreases in the mean values of signaling factors relevant to age were statistically significant in bFGF.

Conclusions. Analyzed data suggest that bFGF, FGFR, IL-6, MMP 8 and 9 were determined as signaling factors in PDL of interradicular septum. Mean expression level decrease with age of FGFR1, IL-6, MMP-8, MMP-9 was non- statistically significant. The mean expression level of bFGF decreased with age, and this decrease was statistically significant. In younger patients, signal molecule expression is higher because of increased PDL metabolic activity. Increased PDL metabolic activity is a reason for higher expression of signal molecule in younger patients. Activity of remodeling process of periodontal tissue decreases with the aging and expression of signaling molecule decreases in adults.

Key words: aging, bFGF, FGFR, MMP-8, MMP-9, IL-6.

Received: 04 07 2011

Accepted for publishing: 22 12 2011

¹Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Riga, Latvia

²Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia

Maris Grzibovskis¹– D.D.S.

Ilga Urtane¹ – D.D.S., PhD, prof.

Mara Pilmane² – PhD, Dr. hab. med., prof.

Address correspondence to: to Maris Grzibovskis, Department of Orthodontics, Dzirciema str. 20, Riga, LV 1007, Latvia.

E-mail address: mgmaris@inbox.lv



	Baltic Dental and Maxillofacial Journal

December, 2011, Vol. 13, No. 4 CONTENTS REVIEWS Dental management of patients before and after renal transplantation Eleni A. Georgakopoulou, Marina D. Achtari, Niki Afentoulide 107-112 Factors influencing face aging. Literature review Kestutis Sveikata, Irena Balciuniene, Janina Tutkuviene 113-116 SCIENTIFIC ARTICLES Specific signaling molecule expression in periodontal ligaments in different age groups: Pilot study Maris Grzibovskis, Ilga Urtane, Mara Pilmane 117-122 Upper airway sagittal dimensions in obstructive sleep apnea (OSA) patients and severity of the disease Juris Svaza, Andrejs Skagers, Dace Cakarne, Iveta Jankovska 123-127 CLINICAL CASE REPORTS Endoscopically assisted enucleation of a large mandibular periapical cyst Heleia Nestal Zibo, Ene Miller 128-131 Squamous cell carcinoma of the left temporal region Albinas Gervickas, Raimundas Golubevas, Alvydas Gleiznys 132-134 © 2011 Stomatologija		Stomatologija 2011; 13 (4): 117-122 454 KB Specific signaling molecule expression in periodontal ligaments in different age groups: pilot study Maris Grzibovskis, Ilga Urtane, Mara Pilmane Summary Introduction. Orthodontic teeth movement is accompanied by remodeling of alveolar bone, including the interradicular septum and periodontal ligaments (PDL). Periodontal signaling molecules have important functions during tooth movement and they are active in the bone remodeling process. Patients involved in orthodontic treatment belong to different age groups: therefore age must be considered as a contributing factor compromising the remodeling potential of periodontal tissues. The aim of the current study was to investigate the specific expression of signaling molecules in the PDL of interradicular septum in patients from different groups of age. Materials and methods. The study group included 25 patients to whom extractions of teeth was recommended as a part of further orthodontic treatment. 25 patients (10 males and 15 females) were divided into three groups as follows: 1) 12-14 years old; 2) 15-22 years old; and 3) 23 years old or older. The routine histological method was followed and samples were stained with hematoxyline-eosine. According to literature data in current immunohistochemical study were included and examined expression of NGFR (nerve growth factor receptor), TGF-β (transforming growth factor β), bFGF (basic fibroblast growth factor), FGFR1 (fibroblast growth factor receptor), IL-1 (interleukin 1), IL-6 (interleukin 6), IL-8 (interleukin 8), MMP-1 (matrix metalloproteinase 1), MMP-2 (matrix metalloproteinase 2), MMP-8 (matrix metalloproteinase 8), MMP-9 (matrix metalloproteinase 9), MMP-13 (matrix metalloproteinase 13) in PDL of interradicular septum. The distribution of these factors was evaluated semi quantitatively. Results. Expression levels of FGFR1, bFGF, MMP 8 and 9, and IL-6 in PDL of interradicular septum structure were determined in all samples. Decreases in the mean values of signaling factors relevant to age were statistically significant in bFGF. Conclusions. Analyzed data suggest that bFGF, FGFR, IL-6, MMP 8 and 9 were determined as signaling factors in PDL of interradicular septum. Mean expression level decrease with age of FGFR1, IL-6, MMP-8, MMP-9 was non- statistically significant. The mean expression level of bFGF decreased with age, and this decrease was statistically significant. In younger patients, signal molecule expression is higher because of increased PDL metabolic activity. Increased PDL metabolic activity is a reason for higher expression of signal molecule in younger patients. Activity of remodeling process of periodontal tissue decreases with the aging and expression of signaling molecule decreases in adults. Key words: aging, bFGF, FGFR, MMP-8, MMP-9, IL-6. Received: 04 07 2011 Accepted for publishing: 22 12 2011 ¹Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Riga, Latvia ²Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia Maris Grzibovskis¹– D.D.S. Ilga Urtane¹ – D.D.S., PhD, prof. Mara Pilmane² – PhD, Dr. hab. med., prof. Address correspondence to: to Maris Grzibovskis, Department of Orthodontics, Dzirciema str. 20, Riga, LV 1007, Latvia. E-mail address: mgmaris@inbox.lv