SCIENTIFIC ARTICLES

In vitro evaluation of cytotoxicity of permanent prosthetic materials
Vaidotas Sabaliauskas, Rima Juciute, Virginija Bukelskiene, Vygandas Rutkunas, Rita Trumpaite-Vanagiene, Alina Puriene
75-80

Specific signaling molecule expressions in the interradicular septum in different age groups
Maris Grzibovskis, Ilga Urtane, Mara Pilmane, Iveta Jankovska
81-86

Soft tissue thickness changes after correcting Class III malocclusion with bimaxillar surgery
Andris Abeltins, Gundega Jakobsone
87-91

Postmenopausal osteoporosis and tooth loss
Anda Slaidina, Una Soboleva, Ilze Daukste, Agnis Zvaigzne, Aivars Lejnieks
92-95

Growth factors, genes, bone proteins and apoptosis in the temporomandibular joint (TMJ) of children with ankylosis and during disease recurrence
Mara Pilmane, Andrejs Skagers
96-101

CLINICAL CASE REPORTS

Focal epithelial hyperplasia: Case report
Alina Puriene, Arunas Rimkevicius, Mindaugas Gaigalas
102-104

Stomatologija 2011; 13 (3): 75-80 297 KB

In vitro evaluation of cytotoxicity of permanent prosthetic materials

Vaidotas Sabaliauskas, Rima Juciute, Virginija Bukelskiene, Vygandas Rutkunas, Rita Trumpaite-Vanagiene, Alina Puriene

Summary

Objectives. To assess qualitative and quantitative cytotoxicity effect on permanent prosthetic materials to human gingival fibroblasts.

Methods. Human gingival tissues were collected (with informed consent) from patients undergoing periodontal surgical procedures and fibroblasts were cultured in vitro. Cell type was determined by performing proteomic analysis. Selected prosthetic materials including titanium, feldspathic ceramic, gold and chrome-cobalt alloy specimens (5×2 mm) were fabricated. The toxicity of prepared specimens was tested by exposing them to cell culture medium for 48, 72, 96 and 120 hours at 37°C under sterile conditions. Cell viability was estimated using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. The data concerning cell viability were statistically analyzed using two-way ANOVA test and Tukey multiple comparison test.

Results. Results obtained after 48 hours showed no toxic effect of titanium compared to control group. Cytotoxic effect was observed in gold alloy and feldspathic ceramic, however, it was not significant compared to control group. Chrome-cobalt alloy significantly reduced cell viability compared to control group (p≤0.001). Cytotoxicity diminished with increasing incubation time of specimens.

After 120 hours of incubation all tested materials, except chrome-cobalt alloy, had no cytotoxicity.

Conclusions. Titanium proved to be non-toxic. Gold alloy and feldspathic ceramic had short-term cytotoxic effect. Chrome-cobalt alloy had highest cytotoxic effect on fibroblast cells.

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¹Institute of Odontology, Faculty of Medicine, Vilnius University, Lithuania

²Vilnius University Zalgirio clinics, Lithuania

³Department of Development Biology, Institute of Biochemistry, Lithuania

Vaidotas Sabaliauskas¹ – D.D.S.

Rima Juciute² – D.D.S.

Vygandas Rutkunas^{1, 2} – D.D.S., PhD, assoc. prof.

Virginija Bukelskiene³ – PhD

Rita Trumpaite-Vanagiene^{1, 2} – D.D.S., assist. prof.

Alina Puriene^{1, 2} – D.D.S., PhD, prof.

Address correspondence to Dr. Vaidotas Sabaliauskas, Institute of Odontology, Faculty of Medicine, Vilnius University, Zalgirio str. 115-219, Vilnius, Lithuania.

E-mail address: vaidas@medicus.lt