Baltic Dental and Maxillofacial Journal
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June, 2009, Vol. 11, No. 2



Evaluation of dental care and the prevalence of tooth decay among middle-aged and elderly population of Kaunas city
Jurate Zubiene, Simona Milciuviene, Jurate Klumbiene

Growth factors/cytokines/defensins and apoptosis in periodontal pathologies
Zane Laurina, Mara Pilmane, Ruta Care

Stress distribution of an internal connection implant prostheses set: A 3D finite element analysis
Regenio Mahfuz Herbstrith Segundo, Hugo Mitsuo Silva Oshima, Isaac Newton Lima da Silva, Luis Henrique Burnnet Junior, Eduardo Goncalves Mota, Liangrid Lutiani Silva

Intra-articular injection of hyaluronic acid for temporomandibular joint osteoarthritis in elderly patients
Luca Guarda-Nardini, Daniele Manfredini, Marco Stifano, Alberto Staffieri, Gino Marioni

Evaluation of tensile strength of different configurations of orthodontic retraction loops for obtaining
optimized forces
Miceli Beck Guimaraes Blaya, Graziela Henriques Westphalen, Magali Beck Guimaraes, Luciana Mayumi Hirakata

© 2009 Stomatologija

Stomatologija 2009; 11 (2): 48-54 413 KB

Growth factors/cytokines/defensins and apoptosis in periodontal pathologies

Zane Laurina, Mara Pilmane, Ruta Care


In the recent past there has been an increased emphasis on morphogenetic tissue research of periodontal tissues. The aim of this study was to find qualitative and quantitative correlations in distribution and appearance of growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Material and methods. Tissue was obtained from 5 controls and 6 chronical periodontitis patients 30-50 years of age referred to Latvian Institute of Stomatology. Histological investigations were performed at the Institute of Anatomy and Anthropology of Riga Stradins University. Results. Epithelial cells abundantly expressed IL10 in patients. The expression of b-defensins was very variable in both sulcular and gingival epithelium. TUNEL positive cells were observed in patients and control specimens with dominance in control group. Gingival epithelium showed moderate expression of bFGF whereas few to moderate cells were positive for bFGF in sulcular epithelium. Fibroblast growth factor receptor (FGF-1R) was abundant in gingival epithelium and in connective tissue cells, but almost not detectable in sulcular epithelium. Insulin-like growth factor receptor was not expressed in gingival epithelium and was weakly seen in basal layer of sulcular epithelium. Basic nerve growth factor expresion in both types of epithelium was numerous to abundant. Staining for the NGFR in the gingival epithelium was variable, with prevalence to be moderate whereas sulcular epithelium was free from any factor immunoreactivity. Conclusion. 1. Finding of apoptotic cells are variable and seems to correlate with the expression of defensins in oral epithelium in patients with periodontitis. 2. FGFR was expressed more than the bFGF, but in case with NGFR and bNGF situation was opposite. Although IGFRI was found in sulcular epithelium with no expression in gingival one suggesting about stimulation in regeneration/adaptation in periodontitis affected tissue. 3. The expression of growth factors and their receptors in sulcular epithelium was lower than into the gingival epithelium and seems to be specific for periodontitis.

Key words: growth factors, cytokines, defensins, apotosis, periodontal health.

Received: 08 11 2008

Accepted for publishing: 19 06 2009

*Riga Stradins University, Riga, Latvia

Zane Laurina* – D.D.S., Department of Periodontology, Institute of Stomatology

Mara Pilmane* – Dr. med., Dr. habil. med., Professor and Director of Institute of Anatomy and Anthropology

Ruta Care* – D.D.S., Professor and Head of Department of Therapeutical dentistry, Institute of Stomatology

Address correspondence to Dr. Zane Laurina, Riga Stradins University, Institute of Stomatology, 20 Dzirciema Street, Riga, Latvia, LV 1007.

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