|Baltic Dental and Maxillofacial Journal|
December, 2007, Vol. 9, No. 4
Finite element analysis of stresses in the maxillary and mandibular dental arches and TMJ articular discs during clenching into maximum intercuspation, anterior and unilateral posterior occlusion
Dynamics of Pregnant Women's Oral Health Status during Preventive Programme
© 2007 Stomatologija
Stomatologija 2007; 9 (4): 99-104 1128 KB
Effect of cyclic 3´, 5´ adenosin-monophosphate on osteogenesis embryonic bone cultivated in tissue culture: An autoradiographic study
Edvitar Leibur, Elbi Lepp
The aim of this study was to evaluate the effect of cyclic 3´,5´adenosin-monophosphate (cAMP) on DNA synthesis of embryonic alveolar bone in tissue culture. Material and methods. Bone fragments were cultured in the medium composed of 80% medium 199, 15% horse serum, 4 mg/ml glucose, 100 mg/ml penicillin using the grid method.The explants were cultured up to 12 days. In the second series, the effect of cAMP in a concentration of 10-6 M on bone during 12 days was studied. DNA synthesis was determined by calculating mitotic labelling indices for 3H-thymidine incorporation into cells within cultured explants at 3 to 12 days. The medium was supplemented with 1 microCurie/ml 3H-thymidine for 4 hours and processed for autoradiography. The mitotic labelling index was determined in the histological sections. All values wer presented as mean±standard deviation. Statistical significance was defined by p-values of 0,05 or less. Results. Morphological and statistical analysis showed that there were differences in mitotic incidence (MI) and silver grain densities over osteoblasts in control cultures and with cAMP. The mean value of MI was 4,627±1,001 in control and 7,706±1,188 in the cultures where cAMP was added (p<0,05). Conclusion. Thus cAMP inhibited bone resorption and stimulated new bone formation in tissue culture. This study provides a novel concept that may help to identify new therapeutic targets.
Key words: autoradiography, cAMP, osteogenesis, tissue culture.
Received: 04 10 2007
Accepted for publishing: 21 12 2007
1Department of Stomatology, Department of Internal Medicine, Tartu University, Estonia
Edvitar Leibur1 MD, PhD, Dr.med.Sc., Dr.h.c. senior researcher, Prof. emeritus
Address correspondence to Dr. Edvitar Leibur, Department of Stomatology, Tartu University Hospital, 8 Puusepa str., Tartu 51014, Estonia.