|Baltic Dental and Maxillofacial Journal|
June, 2007, Vol. 9, No. 2
CLINICAL CASE REPORTS
© 2007 Stomatologija
Growth factors, apoptotic cells and barx1 gene in bone and soft tissue of skeletal class III patients
Iveta Jankovska, Mara Pilmane, Ilga Urtane, Andris Bigestans, Girts Salms, Gunars Lauskis
Growth factors and growth stimulating genes are main signaling molecules for growth and development in ante and postnatal period involved in cellular proliferation, differentiation and morphogenesis of tissues and organs during embryogenesis, postnatal growth and adulthood. The aim of this study was to evaluate TGF-β (transforming growth factor-β), BMP2/4 (bone morphogenetic protein 2/4), FGFR1 (fibroblast growth factor receptor one), barx1 gene and apoptosis from tissue samples of oro-maxillo-facial region in skeletal class III patients to reveal possible morphopathogenesis of severe skeletal anomalies. The study group included 9 patients with skeletal class III malocclusion. During orthognatic surgery tissue samples from tuber maxillae, ramus mandibulae anterior and posterior part, as well as gingiva from the lower jaw in region of second molar have been taken. Samples were stained with immunohistochemistry for TGF-β, BMP2/4, FGFR1, apoptosis and barx1 gene. We used also the routine histological staining with haematoxyline and eosine.
In tuber maxillae, ramus mandibulae anterior and posterior part staining for TGF-β was the most relevant. Also BMP2/4, FGFR1 and barx1 showed the highest mean number of positive cells in tuber maxillae. Barx1 was equally expressed in ramus mandibulae, but BMP2/4 and FGFR1 mainly stained its posterior part cells. Apoptosis mostly affected ramus mandibulae anterior part.
Conclusions. We suggest about more active stimulation of bone growth in tuber maxilla whereas ramus mandibulae. Apoptosis mainly affects ramus mandibulae anterior part that possibly connects to the lower expression of growth stimulating factors and may indicate lower bone remodelation ability.
Key words: growth factors, apoptosis, orthognathic surgery, immuno-histochemistry
Received: 17 04 2007
Accepted for publishing: 10 07 2007
1Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Latvia
2Institute of Anatomy and Anthropology, Riga Stradins University, Latvia
3Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Riga Stradins University, Latvia
Iveta Jankovska1 D.D.S.
Mara Pilmane2 Dr. med., Dr. hab. med., Corresponding member of Latvian Academy of Science, Director of Institute of Anatomy and Anthropology
Ilga Urtane1 D.D.S., Dr. med., Professor and Head of Department of Ortho-dontics, Director of Institute of Stomatology
Andris Bigestans3 M.D., D.D.S., lecturer
Girts Salms3 M.D., D.D.S., lecturer
Gunars Lauskis3 M.D., D.D.S., lecturer
Address correspondence to Dr. Iveta Jankovska, Department of Orthodontics, Institute of Stomatology, Riga Stradins University, 20 Dzirciema Street, Riga, Latvia, LV 1007.