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Baltic Dental and Maxillofacial Journal

December, 2004, Vol. 6, No. 4

CONTENTS

SCIENTIFIC ARTICLES

Magnetic Resonance Imaging Findings of the Temporomandibular Joint Internal Derangement in a Non-patient Population
99 - 102

Assessment of Complete Cleft (CLP) Patients’ Occlusion at Age of Five
103 - 105

Traumatic Damage to the Inferior Alveolar Nerve Sustained in Course of Dental Implantation. Possibility of Prevention
106 - 110

Periodontal Status in Population of Belarus
111 - 113

Modulatory Effect of Ammonium Ions on Human Neutrophil Oxidative Burst in Response to Bacterial Stimuli
114 - 117

Dental Fear Among Teenagers. Individual Anxiety Factors
118 - 121

Damage of Inferior Alveolar Nerve in Mandible Fracture Cases
122 - 125

Stomatologija 2004; 6 (4): 114-117 166 KB

Modulatory Effect of Ammonium Ions on Human Neutrophil Oxidative Burst in Response to Bacterial Stimuli

Nomeda Baseviciene, Pajauta Paipaliene, Irena Nedzelskiene

Summary

Neutrophils (polymorphonuclear leucocytes ) are the principal cells of the host defense system. Consequently, if periodontal pathogen – derived substances in the gingival crevice significantly inhibit their function, they could shift the bacterial – host balance in favour of the bacteria. The aim of investigation was to determine if the ammonium ions (periodontal pathogens produce substantial amounts ) affects the main neutrofils oxidative function – reactive oxygen species generation measured by luminol and lucigene– dependent chemiluminescence. Our results show that ammonium ions both in the group of non-periodontally diseased persons and in periodontitis patients have significant inhibitive effect on luminol- and lucigene-dependent chemiluminescence, which depends on NH4Cl concentration (5 mM, 10 mM, 25 mM, 50 mM), i.e. inhibitive effect increases with increasing concentration of NH4Cl . It shows that the presence of ammonia in the gingival crevice may increase the risk of development of periodontal disease.

Key words: chemiluminescence, lucigene, luminol, neutrophilic leucocytes, ammonium ions

Received: 02 06 2004

Accepted for publishing: 20 12 2004

^*Department of Dental and Oral Pathology, Kaunas Medical University, Kaunas, Lithuania

Nomeda Baseviciene^* - D.D.S., PhD.

Pajauta Paipaliene^* - D.D.S., PhD, assoc. prof., Head of Department of Dental and Oral Pathology.

Irena Nedzelskiene^* - biostatistician.

Address correspondence to dr.Nomeda Baseviciene, Department of Dental and Oral Pathology, Kaunas Medical University, Eiveniu 2, Kaunas, Lithuania.



	Baltic Dental and Maxillofacial Journal

December, 2004, Vol. 6, No. 4 CONTENTS SCIENTIFIC ARTICLES Magnetic Resonance Imaging Findings of the Temporomandibular Joint Internal Derangement in a Non-patient Population 99 - 102 Assessment of Complete Cleft (CLP) Patients’ Occlusion at Age of Five 103 - 105 Traumatic Damage to the Inferior Alveolar Nerve Sustained in Course of Dental Implantation. Possibility of Prevention 106 - 110 Periodontal Status in Population of Belarus 111 - 113 Modulatory Effect of Ammonium Ions on Human Neutrophil Oxidative Burst in Response to Bacterial Stimuli 114 - 117 Dental Fear Among Teenagers. Individual Anxiety Factors 118 - 121 Damage of Inferior Alveolar Nerve in Mandible Fracture Cases 122 - 125 © 2004 Stomatologija		Stomatologija 2004; 6 (4): 114-117 166 KB Modulatory Effect of Ammonium Ions on Human Neutrophil Oxidative Burst in Response to Bacterial Stimuli Nomeda Baseviciene, Pajauta Paipaliene, Irena Nedzelskiene Summary Neutrophils (polymorphonuclear leucocytes ) are the principal cells of the host defense system. Consequently, if periodontal pathogen – derived substances in the gingival crevice significantly inhibit their function, they could shift the bacterial – host balance in favour of the bacteria. The aim of investigation was to determine if the ammonium ions (periodontal pathogens produce substantial amounts ) affects the main neutrofils oxidative function – reactive oxygen species generation measured by luminol and lucigene– dependent chemiluminescence. Our results show that ammonium ions both in the group of non-periodontally diseased persons and in periodontitis patients have significant inhibitive effect on luminol- and lucigene-dependent chemiluminescence, which depends on NH4Cl concentration (5 mM, 10 mM, 25 mM, 50 mM), i.e. inhibitive effect increases with increasing concentration of NH4Cl . It shows that the presence of ammonia in the gingival crevice may increase the risk of development of periodontal disease. Key words: chemiluminescence, lucigene, luminol, neutrophilic leucocytes, ammonium ions Received: 02 06 2004 Accepted for publishing: 20 12 2004 ^Department of Dental and Oral Pathology, Kaunas Medical University, Kaunas, Lithuania Nomeda Baseviciene^ - D.D.S., PhD. Pajauta Paipaliene^* - D.D.S., PhD, assoc. prof., Head of Department of Dental and Oral Pathology. Irena Nedzelskiene^* - biostatistician. Address correspondence to dr.Nomeda Baseviciene, Department of Dental and Oral Pathology, Kaunas Medical University, Eiveniu 2, Kaunas, Lithuania.