|Baltic Dental and Maxillofacial Journal|
September, 2004, Vol. 6, No. 3
© 2004 Stomatologija
Stomatologija 2004; 6 (3): 73-6 205 KB
The Ameloblastous Potentiality of Odontogenous Epithelium Demonstrated in Tissue Culture
Edvitar Leibur1, Tiia Tamme1, Elbi Lepp2
Several theories have been put forward concerning the origin of the neoplastic epithelium in ameloblastoma. Because of the doubt on the possible origin of ameloblastomas from cells with odontogenic potentiality, the aim of the present was to observe the development and growth pattern of odontogenic epithelium and tooth germs in tissue culture. Fourteen molar tooth germs consisting enamel organ and dental papilla of fourteen days old mouse embryos were cultured in a medium on millipore filters. THWP 0.45 μ porosity and 25 ± 5 μ thin. Cultivation was terminated at 21st day. Explants were embedded in cellulose-paraffin for histology. After 2 days cultivation, the epithelial outgrowth of enamel organ was observed, and 2 days later was visible structure resembled the pattern of the dental lamina often seen in ameloblastomas. Histological sections of specimens showed that the outer epithelium has differentiated into a lamellar-like structure and the enamel organ has similar pattern of the ameloblastoma. Formation of microcysts and continued formation of epithelial buds was observed. Tissue culture studies of mouse embryo tooth germs and enamel organ demostrated epithelial proliferation from the borders of odontogenic epithelium and the formation of lamellar-like structure observed in ameloblastomas. This study offers additional experimental evidence that ameloblastomas orginate from epithelium with odontogenic potentiality.
Key-words: Enamel organ, tissue culture, ameloblastomatous potentiality
Received: 09 06 2004
Accepted for publishing: 20 09 2004
1Department of Maxillofacial Surgery, Tartu University Hospital, Tartu , Estonia
2Department of Pathomorphology, Estonian University of Agriculture, Tartu, Estonia
Edvitar Leibur - MD, PhD, Dr.med SC., Dr.h.c., professor and head of the Department of Stomatology, University of Tartu, professor emeritus and consultant at the Department of Maxillofacial Surgery, Tartu University Hospital.
Tiia Tamme - MD, department of maxillofacial surgery of Tartu University Hospital.
Elbi Lepp - VetD, Dr. med. Vet., Docent at the Department of Pathomorphology, Faculty of Veterinary.
Address correspondence to: Prof. Edvitar Leibur, Office: Tartu University Hospital, Department of Maxillofacial Surgery, 8 L.Puusepa Str., 51014 Tartu, Estonia. E-mail: Edvitar.Leibur@kliinikum.ee