|Baltic Dental and Maxillofacial Journal|
2003, Vol. 5, No. 1
© 2003, Stomatologija
Stomatologija 2003; 5 (1): 9-12 184 KB
Electrophoresis of Whole-cell Soluble Proteins of Enterococcus faecalis and Yeast Isolated in the Root Canals of Previously Root-filled Teeth
Vytaute Peciuliene, Irena Balciuniene, Markus Haapasalo
Microbiological samples were taken from the root canals of 40 previously root filled teeth with apical periodontitis requiring retreatment with special emphasis on the occurrence of E. faecalis and C. albicans. Microbes were isolated from 33 out of the 40 teeth. Preliminary characterisation of microbial species found in the root canals was based on aerotolerance, gram staining and production of catalase and indole. Identification to species level was based on motility test and carbohydrate fermentation patterns. The detection of glycosidase and aminopeptidase enzymes was used for identification of facultative gram negative enteric rods. Streptococcal isolates growing on bile-esculin agar plates were further identified to species level by a commercial test panel. Identification of Yeasts was based on cellular appearance in gram stained smears and on production of glycosidase enzymes. Results obtained from the primary tests may give inconsistently expressed phenotypic traits.
In order to confirm the results of primary identification tests, electrophoresis of whole-cell soluble proteins as valuable method for rapid and precise characterisation of oral bacteria was used. This test have showed that the protein patterns of reference strain of E. faecalis and C. albicans exhibited identical or similar characteristics with protein patterns of clinical strains of E. faecalis and C. albicans isolated from the root canals and it confirmed primary tests results.
E. faecalis was found in 21 out of the 33 culture positive root canals. C. albicans was found in 6 out of 33 culture positive root canals.
Key words: E. faecalis, C. albicans, electrophoresis of whole-cell soluble proteins, phenotypic traits
Address correspondence to Dr.Vytaute Peciuliene: 115 Zalgirio str., Vilnius, Lithuania. E-mail: firstname.lastname@example.org
Received: 20 02 2003
Accepted for publishing: 25 03 2003